RNA
Sequencing

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What we offer

Our two main approaches
we focus on total RNA or the RNA-exome covering all coding genes

Universal
total RNA Seq includes all types of RNA (>50bp) and therefore delivers a comprehensive picture of the transcriptome capturing both known and novel features

Breadth of results
Identification of fusion transcripts, differentially expressed genes, alternative splicing, expression profiles, but also SNVs and CNVs

Variety of bioinformatic support
depending on the experimental setup different analysis pipelines might be of interest

Our process

RNA sequencing analyzes the transcriptome and is therefore a quantitative determination of the presence and quantity of a transcribed gene in a biological sample at a given moment. The expression of the transcriptome provides the basis for the identity of a cell and the associated functionality and we are happy to share a couple of details about our workflow with you.

1

Library Preparation

We focus on two different approaches for RNA Seq library prep, sequencing of total RNA or RNA enriched for coding genes:

  • TruSeq Stranded Total RNA (with Ribo-Zero Globin) (Illumina)
  • TruSeq Stranded Total RNA (Illumina)
  • followed by the xGen hybridization capturing of the xGen Exome Research Panel v2 (IDT)

2

Sequencing

At MLLSEQ, sequencing is performed using the Illumina sequencing by synthesis method on the latest generation of sequencing devices, the NovaSeq 6000. Sequencing Coverage is depending on the respective application and research question.

3

Among other things, in this step we provide you with browser-based tools for the comprehensive analysis options:

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