Genome
Sequencing

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Whole genome sequencing WGS aims to read a person’s complete genetic information, detect polymorphisms, somatic mutations, structural variants and copy number changes. In a nutshell: if you want to know everything, WGS is the right choice.

What we offer

High quality
With stringent quality control and high degree of automation we perform WGS in an accredited setting

Breadth of results
Identification of SNVs, Indels, SVs, CNVs and repeat expansion with a singel assay

Variety of library preps
depending on the starting material the right WGS library prep makes the difference

Fast turnaround-time
for a comprehensive assessment and characterization of basically any genome

Our process

We at MLLSEQ developed a unique workflow, that allows us to achieve goals no-one else on the market can currently deliver. It is simple, but highly effcient and we are proud to share a couple of details about it with you.

1

Library Preparation

There are two fundamentally different
approaches for WGS library prep:
PCR-free and with DNA amplification, both with mechanic or enzymatic fragmentation.

  • TruSeq DNA PCR-Free (sonication, PCR-free, Illumina)
  • TruSeq DNA Nano (sonication, amplification, Illumina)
  • Illumina DNA Prep (tagmentation, amplification, Illumina)
  • xGen Prism DNA (for cfDNA and FFPE, no fragmentation, amplification, IDT)

2

Sequencing

At MLLSEQ, sequencing is performed using the Illumina sequencing by synthesis method on the latest generation of sequencing devices, the NovaSeq 6000. Sequencing Coverage is depending on the respective application and research question.

3

Among other things, in this step we provide you with browser-based tools for the interpretation of small and large scale molecular genetic changes. Usually, a tumor-normal comparison is performed in genomic sequencing assays, however our pipeline is additionally used to work with a tumor-unmatched Normal control:

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